FIELD: biotechnology.
SUBSTANCE: described is a sample preparation method for typing major histocompatibility complex genes HLA-A, HLA-B, HLA-C, HLA-DPB1, HLA-DQB1, HLA-DRB1. There are also described pools of oligonucleotide primers for implementation of this method.
EFFECT: development of an efficient method of sample preparation for fragmentary NGS-sequencing of a number of gene regions HLA-A/B/C/DPB1/DQB1/DRB1, allowing to determine alleles of given genes.
10 cl, 9 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR SAMPLE PREPARATION OF HEPATITIS B VIRUS DNA SAMPLES FOR WHOLE GENOME SEQUENCING AND OLIGONUCLEOTIDE PRIMERS FOR ITS IMPLEMENTATION | 2023 |
|
RU2818585C1 |
| NUCLEOTIDE SEQUENCE CODING LYTICASE ENZYME, AND PANEL OF OLIGONUCLEOTIDES FOR OBTAINING SYNTHETIC NUCLEOTIDE SEQUENCE OF LYTICASE GENE | 2023 |
|
RU2826150C1 |
| METHOD OF GENOTYPING TLR2 GENE USING RS3804100 POLYMORPHISM AND A SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES FOR ITS IMPLEMENTATION | 2023 |
|
RU2805860C1 |
| METHOD OF GENOTYPING TLR2 GENE USING RS5743708 POLYMORPHISM AND A SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES FOR ITS IMPLEMENTATION | 2023 |
|
RU2805861C1 |
| METHOD FOR GENOTYPING POLYMORPHIC LOCUS rs1043618 (G>C) OF HSPA1A GENE IN HUMAN BY REAL-TIME PCR USING ALLELE-SPECIFIC FLUORESCENT PROBES | 2024 |
|
RU2828869C1 |
| METHOD OF GENOTYPING TLR1 GENE USING RS5743551 POLYMORPHISM AND A SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES FOR ITS IMPLEMENTATION | 2023 |
|
RU2805859C1 |
| METHOD OF GENOTYPING TLR6 GENE USING RS5743810 POLYMORPHISM AND A SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES FOR ITS IMPLEMENTATION | 2023 |
|
RU2805863C1 |
| METHOD OF GENOTYPING TLR4 GENE USING RS4986790 POLYMORPHISM AND A SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES FOR ITS IMPLEMENTATION | 2023 |
|
RU2805862C1 |
| METHOD OF IDENTIFICATION OF SINGLE-NUCLEOTIDE POLYMORPHISMS IN MULTICOPY GENOME LOCUS USING LOOP-POLYMERASE CHAIN REACTION METHOD WITH SUBSEQUENT HYBRIDIZATION ON BIOLOGICAL MICROCHIP | 2022 |
|
RU2809184C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES FOR SPECIES DIFFERENTIATION OF HUMAN HERPES VIRUS 6A AND HUMAN HERPES VIRUS 6B AND METHOD OF ITS USE | 2022 |
|
RU2806427C1 |
