FIELD: medicine.
SUBSTANCE: group of inventions concerns a test system for chemical compounds screening for inhibitory activity against PARP-1 based on FRET microscopy of single particles (spFRET), which is a mixture comprising a mononucleosome complex on a nucleosome-positioning DNA template labeled with a pair of fluorophores, forming donor-acceptor pairs to implement the FRET effect; PARP-1 protein and its substrate - NAD+. The group of inventions also relates to a method for chemical compounds screening for inhibitory activity against PARP-1 based on FRET microscopy of single particles.
EFFECT: group of inventions provides detection of compounds directed to PARP-1 and capable of inhibiting its activity.
12 cl, 1 ex, 8 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR SCREENING OF ANTITUMOR PREPARATIONS - PARP1 INHIBITORS BASED ON BIOCHEMICAL METHODS OF ANALYSIS | 2015 |
|
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|
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| METHOD AND DEVICE FOR BLOOD PURIFICATION FROM FREE-CIRCULATING DNA | 2018 |
|
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| METHOD FOR DETERMINING THE ACTIVITY OF EXCISION REPAIR ENZYMES OF DNA BASES IN HUMAN CELLS | 2022 |
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