METHODS FOR PURIFYING ANTIBODIES Russian patent published in 2018 - IPC B01D15/38 C07K1/22 B01D15/20 C07K16/00 

FIELD: biochemistry.

SUBSTANCE: method comprises: a) adsorbing a protein to a superantigen immobilised on a solid support; b) removing at least one contaminant by contacting the immobilised superantigen containing the adsorbed protein with a first wash buffer comprising an aliphatic carboxylate, wherein said aliphatic carboxylate comprises a backbone comprising 6-11 carbon atoms; and c) eluting the protein from the superantigen immobilised on the solid support; wherein the protein is selected from the group consisting of a soluble receptor, antibody, antibody fragment, immunoglobulin single variable domain, Fab, F(ab')2, Fv, disulphide linked Fv, scFv, closed conformation multispecific antibody, disulphide-linked scFv or diabody. Also described is a method for purifying a protein from its contaminant-containing solution by chromatography with protein A, comprising: a) balancing the protein A immobilised on a solid phase with a buffer for protein A; b) adsorption of protein from the contaminant-containing solution to protein A immobilised on a solid phase; c) removing at least one contaminant by washing the solid with a first washing buffer for protein A, containing 50 mM or 55 mM tris-base, 45 mM acetic acid, at least one aliphatic carboxylate, at pH 7.2, 7.5 or 8.0, wherein the aliphatic carboxylate is selected from the group consisting of 100 mM sodium caprylate and 20 mM sodium decanoate; and (d) extracting the protein from the solid phase with an elution buffer for protein A, where all the buffers are prepared without the addition of NaCl. In addition, a method for purifying a protein from its contaminant-containing solution by chromatography with a protein L is described, comprising: a) balancing the protein L immobilised on a solid phase with a buffer for protein L; b) adsorption of the protein from the contaminant-containing solution to the protein L immobilised on the solid phase; c) removing at least one contaminant by washing the solid phase with a first wash buffer for protein L containing 52 mM tris-base, 48 mM acetic acid, at least one aliphatic carboxylate, at pH 7.0 where the aliphatic carboxylate is selected from the group consisting of 100 mM sodium caprylate and 20 mM sodium decanoate; and (d) extracting the protein from the solid phase with an elution buffer for protein L, where all the buffers are prepared without the addition of NaCl.

EFFECT: described is a method for purifying a protein from a solution containing at least one contaminant by affinity chromatography with a superantigen.

38 cl, 3 dwg, 8 tbl, 7 ex