FIELD: biotechnology.
SUBSTANCE: invention relates to a method for purifying recombinant polypeptide from host cell peptides (hereinafter – HCP), the method includes: (a) applying a solution containing recombinant polypeptide and HCP to a super-antigenic chromatographic solid carrier, (b) washing the super-antigenic chromatographic solid carrier with a washing buffer containing at least 75 mM of caprylate and more than 0.5 M of arginine; and (c) eluting recombinant polypeptide from the super-antigenic chromatographic solid carrier.
EFFECT: method shows an increase in HCP removal.
20 cl, 13 dwg, 9 tbl, 8 ex
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