FIELD: chemistry.
SUBSTANCE: invention relates to the isolation and purification of nucleic acids and fragments thereof from biological samples. Magnetic sorbent for isolating and purifying nucleic acids is proposed, which is an aqueous dispersion containing a composite material, which is a nanoparticle of magnetite doped with cations of copper, zinc and cobalt. Nanoparticles are distributed in a silica matrix. Dispersion also contains a chaotropic agent and a reagent for increasing the ionic strength of the solution.
EFFECT: technical result is to increase the efficiency of magnetic isolation of nucleic acids, to increase the selectivity of sorption of nucleic acids and / or nucleic acid fragments longer than 500 bp.
19 cl, 4 tbl, 9 ex
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| COMPOSITE NANO-SIZE MATERIAL FOR DNA/RNA ADSORPTION AND DESORPTION | 2006 |
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| REPLACEABLE MICROFLUID MODULE FOR AUTOMATED RECOVERY AND PURIFICATION OF NUCLEIC ACIDS FROM BIOLOGICAL SAMPLES AND METHOD FOR RECOVERY AND PURIFICATION NUCLEIC ACIDS WITH USING THEREOF | 2008 |
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| METHOD FOR EXTRACTING CIRCULATING DNA FROM PLASMA OR BLOOD SERUM | 2015 |
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| METHOD OF ISOLATION AND PURIFICATION OF NUCLEIC ACIDS | 1996 |
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| METHOD OF SEPARATING AND PURIFYING NUCLEIC ACIDS | 2008 |
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| MAGNETIC COMPOSITE SORBENT | 2012 |
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| METHOD FOR ISOLATION OF NUCLEIC ACIDS | 2004 |
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| AUTOMATED DEVICE FOR ISOLATION, PURIFICATION AND ANALYSIS OF NUCLEIC ACIDS BY RT-PCR METHOD | 2020 |
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