FIELD: biochemistry.
SUBSTANCE: invention relates to biochemistry. Method for estimating the biotropic manifestation of ultrahigh-frequency electromagnetic radiation is described, integrated under the control of the dps gene, according to which the regulatory region of the dps gene is integrated into the pET28b-EGFP plasmid in front of the GFP reporter protein gene, E. coli cells are transformed with the resulting plasmid; in this case, an increase in the intensity of the fluorescent radiation of the transformed bacterial cells, recorded visually, indicates an increased level of electromagnetic radiation of the microwave range, which acts on the cells. Results obtained with this method allow to use the regulatory region of the dps gene in biosensors that track the background level of electromagnetic radiation.
EFFECT: invention expands the range of means for assessing the biotropic manifestation of electromagnetic radiation.
1 cl, 2 dwg
Title |
Year |
Author |
Number |
BACTERIAL STRAIN ESCHERICHIA coli/pTdcR-turboYFP, HAVING SENSITIVITY TO TERAHERTZ RADIATION |
2018 |
- Serdyukov Danil Sergeevich
- Goryachkovskaya Tatyana Nikolaevna
- Rozanov Aleksej Sergeevich
- Meshcheryakova Irina Anatolevna
- Peltek Sergej Evgenevich
|
RU2691308C1 |
STRAIN OF ESCHERICHIA COLI BACTERIA FOR CHECKING PRESENCE OF PHENOL AND HYDROPEOXIDE IN MEDIUM |
2007 |
- Kachko Alla Vasil'Evna
- Tikunova Nina Viktorovna
- Khlebodarova Tamara Mikhajlovna
- Kolchanov Nikolaj Aleksandrovich
|
RU2348687C1 |
GENE-THERAPEUTIC DNA-VECTOR GDTT1.8NAS12, METHOD FOR ITS PREPARATION, ESCHERICHIA COLI JM110-NAS STRAIN, A METHOD FOR ITS PREPARATION, ESCHERICHIA COLI JM110-NAS/GDTT1.8NAS12 STRAIN, CARRYING A GENE-THERAPEUTIC DNA-VECTOR GDTT1.8NAS12, A METHOD FOR PRODUCTION THEREOF, A METHOD FOR INDUSTRIAL PRODUCTION OF A GENE-THERAPEUTIC DNA VECTOR |
2018 |
|
RU2712838C1 |
NON-REPLICATIVE TRANSDUCTION PARTICLES AND TRANSDUCTION PARTICLE BASED REPORTER SYSTEMS |
2014 |
- Rey Diego Ariel
- Deforest Nikol
- Cox Heather
- Shukla Soni
|
RU2661101C2 |
RECOMBINANT PLASMID DNA phPGK-eGFP-CATTCGCC-CMV-mCherry, PROVIDING HIGH EXPRESSION OF REPORTER PROTEIN eGFP IN CULTURED CHINESE HAMSTER OVARY CELLS |
2023 |
- Omelina Evgeniya Sergeevna
- Andreeva Evgeniya Nikolaevna
- Boldyreva Lidiya Valerevna
|
RU2822596C1 |
VTVAF17 GENE-THERAPY DNA VECTOR, METHOD FOR PRODUCING THEREOF, ESCHERICHIA COLI SCS110-AF STRAIN, METHOD FOR PRODUCING THEREOF, ESCHERICHIA COLI SCS110-AF/VTVAF17 STRAIN, BEARING VTVAF17 GENE-THERAPY DNA VECTOR, METHOD FOR PRODUCING THEREOF |
2017 |
|
RU2678756C1 |
METHOD FOR DETECTION OF COPPER IONS IN THE ENVIRONMENT AND BIOSENSOR FOR ITS IMPLEMENTATION |
2015 |
- Nazarov Aleksej Vladimirovich
- Otroshchenko Anatolij Anatolevich
- Korsakova Ekaterina Sergeevna
- Boronnikova Svetlana Vitalevna
- Makarikhin Igor Yurevich
- Shumkov Mikhail Sergeevich
|
RU2628704C2 |
NUCLEIC ACID HAVING PROMOTER ACTIVITY AND ITS USE |
2022 |
- Vlasova Elena Veniaminovna
- Strelkova Anna Nikolaevna
- Tyshchuk Konstantin Ilich
- Gershovich Pavel Mikhailovich
- Perepelkina Mariya Pavlovna
- Iakovlev Pavel Andreevich
- Morozov Dmitry Valentinovich
|
RU2818112C2 |
MICROVESICLES ORIGINATING FROM CELL PROTOPLASTS AND THEIR USE |
2011 |
- Gkho Jong Song
- Dzang Su Chul
- Kim Joon Keun
- Kim Okh Ioun
|
RU2570636C2 |
POLYNUCLEOTIDE, CODING CHROMO- OR FLUORESCENT MUTANT POLYPEPTIDE, EXPRESSION VECTOR, CELL, METHOD OF OBTAINING CHROMO- OR FLUORESCENT POLYPEPTIDE, USE OF POLYPEPTIDE AND USE OF POLYNUCLEOTIDE |
2002 |
- Bevis Bruk
- Glik Bendzhamin
|
RU2330067C2 |