METHOD FOR ELECTROIMOBILIZATION OF ANTIBODIES Russian patent published in 2018 - IPC C07K17/14 C12N15/13 G01N27/00 

Abstract RU 2658350 C1

FIELD: biochemistry.

SUBSTANCE: invention relates to biochemistry. Method for electroimobilization of antibodies is proposed. Method involves applying constant electric field energy through flat electrodes from a chemically inactive metal to an electrolytic buffer solution of antibodies. Moreover, the antibody solution in tris-acetate buffer is placed in a vertically disassembled microlectrophoretic chamber filled with granules of silica gel 40, and the particles of a standard sample of magnetic sorbent are fixed with niobium magnets. Electrodes of the chamber are quartz resonators with gold deposition. Supplied calibrated electric field with a constitutive specific power relative to the cross-sectional area of the chamber is not more than 2.9 W/cm2.

EFFECT: invention provides a reduction in time and an increase in the effectiveness of antibody immobilization, as well as the saving of antibody solutions.

6 cl, 8 ex

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Authors

Kalnoj Sergej Mikhajlovich

Kulichenko Aleksandr Nikolaevich

Afanasev Evgenij Nikolaevich

Tyumentseva Irina Stepanovna

Kurcheva Svetlana Aleksandrovna

Startseva Olga Leonidovna

Geogdzhayan Anna Samvelovna

Kovalev Dmitrij Anatolevich

Selimov Magomed Aslanovich

Yanovich Sergej Vladimirovich

Dates

2018-06-20Published

2017-02-02Filed