FIELD: biotechnology.
SUBSTANCE: attenuated influenza vector induces a cross-protective response against influenza A and B viruses containing its pharmaceutical and vaccine compositions and their use. The characterized attenuated influenza vector contains the nucleotide sequences of the genes of the proteins PB2, PB1, PA, NP and M, originating from the influenza virus A/PR/8/34 (H1N1), the nucleotide sequences of the HA and N protein genes originating from the influenza A/California/7/09-like (H1N1pdm), a nucleotide sequence of a chimeric NS protein gene comprising a truncated reading frame of the 124 amino acid NS1 gene derived from the A/PR/8/34 (H1N1) virus and continued with insertion of the nucleotide sequence encoding the HA2 subunit fusion peptide From the virus gr Ipp B, and the nucleotide sequence encoding the conserved B-cell epitope of the influenza A virus nucleoprotein (TK), and the sequence of the Nep protein gene derived from the A/Singapore/1/57-like (H2N2) virus.
EFFECT: inventions can be used to prevent influenza A and B.
16 cl, 15 dwg, 4 ex
