FIELD: genetic engineering, virology.
SUBSTANCE: invention proposes a method for preparing the attenuated recombinant strain of the influenza virus A.Method involves preparing the recombinant NS segment of influenza virus A comprising the functional RNA-binding domain and modification of its gene sequence below nucleotide 400 in the gene NS1. This modification prevents translation of the remaining moiety of the gene NS1. Also, invention proposes the genetic-engineering strain of influenza virus A, vaccine comprising thereof, and the recombinant segment of the gene NS of influenza virus A. The prepared genetic engineering strain of influenza virus A possesses the interferon-inducing effect and retains the interferon-nonsensitive natural phenotype and able to induce response as production of interferon in MDCK cells and in fertilized chicken eggs, and to grow in latter. Invention can be used in medicine and virology.
EFFECT: improved and valuable properties of recombinant virus.
17 cl, 20 dwg, 6 ex
