METHOD FOR SIMULATING IATROGENIC BILE DUCT INJURIES IN EXPERIMENTAL ANIMAL Russian patent published in 2018 - IPC G09B23/28 A61B17/94 

FIELD: medicine.

SUBSTANCE: invention relates to experimental medicine and can be used for simulating iatrogenic bile duct injuries (IBDI) in pigs. Simulation is carried out in a pig. Method comprises sedation of an animal, fixation on the laboratory table in the supine position with spread out and fixed limbs, anesthesia and infusion therapy. Carboxy-peritoneum is further formed with Veres needle over the umbilicus with a partial pressure of carbon dioxide of 14 mm Hg at conditions of combined general anesthesia with intubation of the trachea and artificial ventilation of the lungs. 4 trocars are introduced for manipulators: above the umbilicus – a trocar with a diameter of 10 mm, under the ensisternum – 2 trocars with a diameter of 5 mm at a distance of 3 cm from each other, and in the right hypochondrium region – a trocar with a diameter of 5 mm. Then, with the help of a soft clamp and a dissector, a choledochous duct is separated from the hepatoduodenal ligament at the point of cystic duct confluence into the common hepatic duct. Clipator with a diameter of 10 mm is inserted through a separate puncture of the anterior abdominal wall under the ensisternum with a diameter of 10 mm. Choledochous is clipped, with fixation of 2 clips in the proximal direction from the point of cystic duct confluence and of 1 clip in the distal direction. Choledochous duct is further completely transected below the point of confluence into the cystic duct, between the 2 proximal clips and the 1 distal clip. In case of mechanical damage, the choledochous duct is transected by means of endoscopic scissors. In case of thermal damage, it is transected by means of an L-shaped electrode for dissection under the action of a monopolar diathermic generator, installed in dissection mode at a given current strength of 70A for all diathermic influences.

EFFECT: proposed original model while minimizing the trauma and the magnitude of blood loss is comparable with the IBDI in humans when performing laparoscopic cholecystectomy, which allows conducting experimental studies aimed at the reconstruction and/or restoration of the damaged duct.

1 cl, 4 dwg