FIELD: biotechnology.
SUBSTANCE: invention relates to applied microbiology, biotechnology and the microbiological industry. Method for the biosynthesis of the nuclease of Serratia marcescens bacteria involves the addition of mitomycin C in an amount of 0.01–1.0 mg/l of the culture liquid during the exponential growth of Serratia marcescens bacteria on LB medium. In this case, the culture is carried out 6–22 hours with mitomycin C under conditions of a high degree of aeration of the medium in the range 340–870 mg O2/l/hour, which is achieved by aerating it with deep cultivation with increasing temperature up to 37 °C.
EFFECT: invention provides an increase in the intensity of the biosynthesis of nuclease by the Serratia marcescens bacteria.
1 cl, 5 dwg, 1 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| PHARMACEUTICAL COMPOSITION FOR IMPROVING RHEOLOGICAL PROPERTIES OF SPUTUM BASED ON ENZYME NUCLEASE SERRATIA MARCESCENS | 2018 |
|
RU2695132C1 |
| STRAIN OF BACTERIA MARCESCENS 24 PRODUCER OF ENDONUCLEASE | 0 |
|
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| RECOMBINANT SERRATIA MARCESCENS NUCLEASE PROTEIN FOR HYDROLYSIS OF NUCLEIC ACIDS | 2022 |
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| METHOD OF PRODIGIOSIN PRODUCTION | 2007 |
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| ANTIVIRAL AGENT BASED ON SERRATIA-FAMILY BACTERIA NUCLEASE | 2014 |
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| STRAIN OF BACTERIA Serratia plymuthica HAVING ANTIVIRAL ACTIVITY AGAINST INFLUENZA VIRUS OF TYPE A (VERSIONS) | 2013 |
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| METHOD FOR CULTURING MICROORGANISM SERRATIA MARCESCENS - PRODUCER OF CHITINASE | 2003 |
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RU2241744C2 |
| CULTURE MEDIUM FOR GROWING SERRATIA MARCESCENS 24 PRODUCING NUCLEASE | 0 |
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| METHOD FOR PRODUCING SERRATIA MARCESCENS RECOMBINANT ENDONUCLEASE | 2021 |
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RU2795623C2 |
