METHOD FOR PRODUCING RECOMBINANT PROTEIN OF SERRATIA MARCESCENS BACTERIA NUCLEASE FOR HYDROLYSIS OF NUCLEIC ACIDS Russian patent published in 2022 - IPC C12N9/22 C12N15/31 C12N15/52 C12P21/02 C07K1/16 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology, specifically, to recombinant production of enzymes in E. coli, and can be used to produce recombinant protein of Serratia marcescens nuclease. The method includes producing an oligonucleotide duplex encoding the corresponding gene SEQ ID NO: 2 S. marcescens, optimised for expression in E. coli; creating a plasmid containing the sequence of the gene SEQ ID NO: 2 of S. marcescens nuclease, and producing a producer strain; growing producer cells of the E. coli strain, expressing the gene of S. marcescens nuclease without a signal sequence, in an inactive, non-toxic and insoluble form - as inclusion bodies within bacterial cells; destructing the created producer cells, isolating, washing the inclusion bodies from strain proteins to produce the finished product; further regenerating the resulting Serratia marcescens nuclease to the native conformation, and purifying.

EFFECT: invention provides a high level of nuclease biosynthesis in E. coli, allows for a reduction in the time of the process cycle of nuclease purification with an increase in the output of the target protein.

1 cl, 6 ex