METHOD FOR DETERMINATION OF PREPARATION INFLUENCE ON INTERACTION OF COMPLEMENT WITH ANTIGEN-ANTIBODY COMPLEX Russian patent published in 2018 - IPC G01N33/53 

FIELD: chemistry.

SUBSTANCE: invention relates to medicine, namely biological chemistry, and can be used to determine the effect of drugs on the classical pathway of complement activation by studying the hemolytic activity of complement. To do this, 50 mcl of blood serum from ½ to 1/128 of blood serum are added to 50 mcl each before dilution in a saline solution to a concentration of 10^-2 M, preliminary incubation is carried out at a temperature of 4–6 °C for 30 minutes, then add 50 mcl of sensitized erythrocytes of ram and veronal buffer. Then the cells are re-incubated, the supernatant is taken out and its optical density is determined at 450 nm, and complement fixation (C%) with sensitized erythrocytes in the presence of the studied preparations is calculated by the formula: C%=(E_o-E_c)/E_o-100, where: E_o – optical density in the presence of the preparation, E_c – the optical density of the control, which is used as a veronal buffer. Value of C is judged on the effect of the drug on the C1q subcomponent of the first component of the classical complement pathway in terms of the degree of hemolysis of the sensitized erythrocytes of the sheep.

EFFECT: use of this method makes it possible to make a comparative quantitative assessment of the degree of influence of the tested drugs on the classical pathway of complement activation.

1 cl, 2 dwg, 2 ex