FIELD: medicine, biochemistry. SUBSTANCE: parathyroid animal glands were homogenized in acetic acid solution at ratio tissue : acid = (1:8)-(1: 10) at zinc chloride addition at amount 1 g/1 l mixture at pH = 3-3.5, at +4-8 C for 36-60 hr. Extract is filtered, filtrate is treated with acetone at ratio 1: 5 at -5 - +5C for 18-22 h. Obtained precipitate containing the end product is dissolved in distilled water, filtered on zeolite and lyophilized. EFFECT: simplified method, increased yield of protease inhibitor.
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Authors
Dates
1996-10-20—Published
1990-07-17—Filed