FIELD: biochemistry.
SUBSTANCE: group of inventions relates to biochemistry. Proposed is a nanoparticle for cell transfection, as well as a method for modifying intracellular polynucleotides. Said nanoparticle includes a polyplex core and a silicon dioxide coating on it, wherein the core of the polyplex contains one or more anionic polymers, one or more cationic polymers, a histone terminal peptide, a cationic polypeptide, and a polynucleotide. This method for modifying intracellular polynucleotides involves the contact of a cell with a nanoparticle.
EFFECT: inventions provide for the production of nanoparticles with improved stability, directed delivery to specific cell types, greater nuclear specificity, and compartment-specific unpacking, improved ability to maintain a significant load level in the initial stages of internalization and the ability to support load release after internalization.
22 cl, 26 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PRODUCING PLASMID DNA IN POLYMER NANOSPHERES FOR DELIVERY | 2022 |
|
RU2817116C1 |
| HYBRID CARRIERS FOR DELIVERY OF NUCLEIC ACIDS | 2017 |
|
RU2751001C2 |
| METHODS AND COMPOSITIONS FOR TREATING MULTIPLE SCLEROSIS AND RELATED DISORDERS | 2013 |
|
RU2675322C2 |
| NEW VERTEBRATE CELLS AND METHODS OF RECOMBINANT EXPRESSION OF POLYPEPTIDE OF INTEREST | 2015 |
|
RU2710726C2 |
| GENE EDITING USING MODIFIED CLOSED-END DNA (ceDNA) | 2018 |
|
RU2811724C2 |
| COMPOSITION OF ANTIGEN-MHC NANOPARTICLES FOR INDUCING AN ANTI-INFLAMMATORY RESPONSE IN THE INTESTINE AND A METHOD FOR PRODUCTION THEREOF | 2013 |
|
RU2689563C2 |
| INSERTION OF TARGET GENE FOR IMPROVED CELLULAR IMMUNOTHERAPY | 2017 |
|
RU2824204C2 |
| METHODS AND COMPOSITIONS FOR TREATING HEMOPHILIA B | 2011 |
|
RU2608643C2 |
| POLYCONJUGATES FOR POLYNUCLEOTIDE INTRODUCTION IN VIVO | 2007 |
|
RU2430740C2 |
| COMPOSITIONS CONTAINING LIPASES, AND METHODS FOR SURFACE TREATMENT | 2013 |
|
RU2612215C2 |
