NEW VERTEBRATE CELLS AND METHODS OF RECOMBINANT EXPRESSION OF POLYPEPTIDE OF INTEREST Russian patent published in 2020 - IPC C12N15/09 C12N15/113 C12N5/10 C12N9/50 C12P21/02

Abstract RU 2710726 C2

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, specifically to production of isolated mammal cells suitable for recombinant expression of polypeptide of interest, where the mammalian cell is altered to deteriorate the function of the endogenous protease of the mathematase by knockout, mutation, deletion or silencing of the matrix gene.

EFFECT: use of the obtained mammalian cells to obtain the recombinant polypeptide of interest significantly reduces clipping of the polypeptide of interest secreted in the cell culture medium.

15 cl, 9 dwg, 14 tbl, 6 ex

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RU 2 710 726 C2

Authors

Laux, Holger

Romand, Sandrine

Bodendorf, Ursula

Dates

2020-01-10—Published

2015-04-29—Filed