FIELD: chemistry.
SUBSTANCE: present invention relates to a method for the preparation of a synthetic fissurella hemocyanin (KLH). Method of producing fissurella hemocyanin (KLH) comprising the steps of: a) dissociating native KLH to obtain sub-units, b) nanofiltrating the sub-units obtained in step a) using filters with a pore size between 15 and 35 nm; and c) associating the sub-units obtained after nanofiltering to obtain the synthetic KLH. Method of producing immunocyanin. Synthetic fissurella hemocyanin (KLH), obtained by the above method. Synthetic immunocyanin obtained by the above method. Method of removing virus from a hemocyanin preparation.
EFFECT: described method allows obtaining synthetic hemocyanin and immunocyanin of fissurella, with a high purification degree.
20 cl, 6 ex
Title | Year | Author | Number |
---|---|---|---|
PREPARATION METHODS FOR A NOVEL GENERATION OF BIOLOGICAL SAFE KLH PRODUCTS USED FOR CANCER TREATMENT, FOR THE DEVELOPMENT OF CONJUGATED THERAPEUTIC VACCINES AND AS CHALLENGING AGENTS | 2014 |
|
RU2681413C2 |
METHOD OF TREATMENT OF IFN-ALPHA-CONNECTED STATES | 2012 |
|
RU2639135C2 |
SYNTHETIC PEPTIDE FOR PREPARING MONOSPECIFIC ANTIBODIES AGAINST ISOFORM 2 OF EUKARYOTIC TRANSLATION ELONGATION FACTOR 1A | 2013 |
|
RU2562880C2 |
METHOD OF PRODUCING IMMUNOGLOBULINS | 2016 |
|
RU2708394C2 |
METHOD OF ALPHA-1-ANTITRYPSIN SOLUTION TREATMENT | 2004 |
|
RU2370500C2 |
COMPOSITIONS OF CARBOHYDRATE VACCINE FOR INDUCING IMMUNE RESPONSES AND USES THEREOF IN CANCER TREATMENT | 2014 |
|
RU2666141C2 |
METHOD FOR PREPARING HIGH-TEMPERATURE STABLE PHARMACEUTICAL COMPOSITION CONTAINING OXYGEN CARRIER AND USING IT | 2011 |
|
RU2475252C1 |
FULL FLOW-THROUGH PROCESS FOR PURIFYING RECOMBINANT PROTEINS | 2019 |
|
RU2792435C2 |
IMPROVING IMMUNOGLOBULIN AFFINITY CHROMATOGRAPHY BY THE APPLICATION OF PRE-CAPTURE FLOCULATION | 2020 |
|
RU2794431C1 |
CONJUGATES OF PEPTIDE Melan-A, VIRUS-LIKE PARTICLE ANALOGUE | 2004 |
|
RU2351362C2 |
Authors
Dates
2018-11-12—Published
2014-06-12—Filed