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2 676 099

(13)

(51)

IPC

G01N33/569(2006-01-01)

G01N33/58(2006-01-01)

C12Q1/68(2006-01-01)

(21) (22)

Application

2017139641, 2017-11-14

(24)

Start date

2017-11-14

(22)

Actual filing date

2017-11-14

(45)

Published

2018-12-26

(72)

Inventor

Syromyatnikov Mikhail YurevichKiryanova Svetlana VladimirovnaPopov Vasilij Nikolaevich

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Obrazovatelnoe Uchrezhdenie Vysshego Obrazovaniya Gosudarstvennyj Universitet" Vo

METHOD FOR IDENTIFICATION OF YEAST GENUS PICHIA BASED ON REAL TIME PCR USING A TAQMAN PROBE Russian patent published in 2018 - IPC G01N33/569 G01N33/58 C12Q1/68

Abstract RU 2676099 C1

FIELD: biology.

SUBSTANCE: invention relates to microbiology and is intended for identification of yeast of the genus Pichia. Pre-enrichment of yeast, precipitation by centrifugation, isolation of DNA with real time PCR are carried out. For amplification, primers and a Taqman probe are used. Logarithmic increase in the level of fluorescence in the channel of the FAM amplifier indicates the presence of a yeast strain of the genus Pichia.

EFFECT: invention provides increase in sensitivity of the method and a shorter time for the analysis of the presence of the yeast of the genus Pichia.

1 cl, 1 dwg, 1 ex

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RU 2 676 099 C1

Authors

Syromyatnikov Mikhail Yurevich

Kiryanova Svetlana Vladimirovna

Popov Vasilij Nikolaevich

Dates

2018-12-26—Published

2017-11-14—Filed