FIELD: biology.
SUBSTANCE: invention relates to microbiology and is intended for identification of yeast of the genus Pichia. Pre-enrichment of yeast, precipitation by centrifugation, isolation of DNA with real time PCR are carried out. For amplification, primers and a Taqman probe are used. Logarithmic increase in the level of fluorescence in the channel of the FAM amplifier indicates the presence of a yeast strain of the genus Pichia.
EFFECT: invention provides increase in sensitivity of the method and a shorter time for the analysis of the presence of the yeast of the genus Pichia.
1 cl, 1 dwg, 1 ex
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