Show metadata Hide metadata

(19)

(11)

2 677 332

(13)

(51)

IPC

C12N1/00(2006-01-01)

(21) (22)

Application

2017140038, 2017-11-17

(24)

Start date

2017-11-17

(22)

Actual filing date

2017-11-17

(45)

Published

2019-01-16

(72)

Inventor

Savinova Tatyana StepanovnaSavinova Olga SergeevnaVavilova Ekaterina AleksandrovnaVasina Darya VladimirovnaTyazhelova Tatyana VladimirovnaFedorova Tatyana VasilevnaChulkin Andrej MikhajlovichSolev Pavel Nikolaevich

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Obrazovatelnoe Uchrezhdenie Vysshego Obrazovaniya Gosudarstvennyj Universitet Imeni M.V. Lomonosova"

RECOMBINANT STRAIN OF FILAMENTOUS FUNGUS ASPERGILLUS NIDULANS AND ITS USE FOR PROGESTERONE 11Α-HYDROXYLATION AND FOR ACETYLATION OF 11Α-HYDROXYPROGESTERONE Russian patent published in 2019 - IPC C12N1/00

Abstract RU 2677332 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and biochemistry, namely to recombinant mycelial fungus Aspergillus nidulans 031/pDHG25-SgrDI (pyrG^-) strain. This strain has steroid-11α-hydroxylating activity, the ability to convert progesterone into 11α-acetoxyprogesterone, acetylating activity and ability to esterify 11α-hydroxyprogesterone. Present strain was obtained by transforming strain A. nidulans 031 (argB^-; pyrG^-) (AN031;CBS 129193) autonomously replicable plasmid pDHG25-SgrDI containing a unique SgrDI restriction site. Invention also relates to use of said strain for 11α-hydroxylation of progesterone to form 11α-hydroxyprogesterone and for acetylation 11α-hydroxyprogesterone to give 11α-acetoxyprogesterone.

EFFECT: present invention allows to obtain 11α-hydroxyprogesterone and 11α-acetoxyprogesterone with the help of biocatalytic processes.

3 cl, 23 dwg, 1 tbl, 6 ex

Similar patents RU2677332C1

Title	Year	Author	Number
BIOCATALYST FOR PRODUCING 11α-ACETOXYPROGESTERONE	2017	Savinova Tatyana Stepanovna Savinova Olga Sergeevna Vavilova Ekaterina Aleksandrovna Vasina Darya Vladimirovna Tyazhelova Tatyana Vladimirovna Fedorova Tatyana Vasilevna Chulkin Andrej Mikhajlovich Solev Pavel Nikolaevich	RU2692932C2
MICROBIOLOGICAL METHOD OF PRODUCING 11Α-ACETOXYPROGESTERONE	2018	Savinova Tatyana Stepanovna Savinova Olga Sergeevna Solev Pavel Nikolaevich Vasina Darya Vladimirovna Tyazhelova Tatyana Vladimirovna Fedorova Tatyana Vasilevna	RU2731712C2
METHOD OF DELTA-3-KETOSTEROIDS 11 BETA-HYDROXYLATION	2008	Andrjushina Valentina Aleksandrovna Vojshvillo Natalija Evgen'Evna Druzhinina Anna Viktorovna Stytsenko Tat'Jana Semenovna Jaderets Vera Vladimirovna Skrjabin Konstantin Georgievich Bartoshevich Jurij Ehduardovich Novak Marina Iogannovna Domracheva Alla Georgievna	RU2399674C2
N-SUBSTITUTED DERIVATIVES OF 6α-PIPERAZINOMETHYLANDROOST-4-EN-3,17-DIONE, HAVING ANTIMICROBIAL ACTIVITY, AND THEIR PRODUCTION METHOD	2023	Sergeev Vladimir Nikolaevich Davydova Nadezhda Konstantinovna Sadykova Vera Sergeevna Savinova Tatyana Stepanovna	RU2807924C1
METHOD FOR PREPARING 14α-HYDROXYDERIVATIVES OF Δ-3,17-DIKETO-ANDROSTENE	2009	Jaderets Vera Vladimirovna Andrjushina Valentina Aleksandrovna Vojshvillo Natalija Evgen'Evna Dvojnikov Pavel Stanislavovich Druzhinina Anna Viktorovna Stytsenko Tat'Jana Semenovna Zejnalov Orkhan Akhmedovich Skrjabin Konstantin Georgievich	RU2407800C1
METHOD FOR PREPARING 6α-METHYLHYDROCORTISONE	2006	Lukashev Nikolaj Vadimovich Kazantsev Aleksej Vital'Evich Savinova Tat'Jana Stepanovna Arinbasarova Anna Jur'Evna	RU2297423C1
METHOD OF OBTAINING 11BETA, 17 ALPHA, 21-TRIHYDROXY-16ALPHA-METHYL-9ALPHA-FLUOROPREGNA-1,4-DIENE-3,20-DIONE (DEXAMETHAZONE) FROM PHYTOSTEROL	2013	Kazantsev Aleksej Vital'Evich Alexey Vitalievich) Savinova Tat'Jana Stepanovna Tatyana Stepanovna) Lukashev Nikolaj Vadimovich Nikolay Vadimovich) Dovbnja Dmitrij Vladimirovich Dmitry Vladimirovich) Khomutov Sergej Mikhajlovich Sergei Mikhailovich) Sukhodol'Skaja Galina Viktorovna Galina Viktorovna) Shutov Andrej Anatol'Evich Andrei Anatolievich) Fokina Viktorija Valer'Evna Vistoria Valerievna) Nikolaeva Vera Maksimovna Vera Maximovna) Donova Marina Viktorovna Marina Viktorovna) Egorova Ol'Ga Valer'Evna Olga Valerievna) Surovtsev Viktor Vasil'Evich Viktor Vasilievich)	RU2532902C1
METHOD FOR OBTAINING 6-DEHYDRO-6-METHYLHYDROCORTISONE OR ESTER THEREOF FROM HYDROCORTISONE 21-ACETATE	2017	Savinova Tatyana Stepanovna Kazantsev Aleksej Vitalevich Lukashev Nikolaj Vadimovich	RU2663893C1
METHOD OF PRODUCING 6-METHYLENEANDROST-4-ENE-3,17-DIONE FROM ANDROST-4-ENE-3,17-DIONE, METHOD OF PRODUCING 6-METHYLENEANDROST-1,4-DIENE-3,17-DIONE (EXEMESTANE) USING OBTAINED 6-METHYLENEANDROST-4-ENE-3,17-DIONE	2010	Savinova Tat'Jana Stepanovna Lukashev Nikolaj Vadimovich Latyshev Gennadij Vladimirovich Sukhodol'Skaja Galina Viktorovna Donova Marina Viktorovna Fokina Viktorija Valer'Evna Shutov Andrej Anatol'Evich Nikolaeva Vera Maksimovna Surovtsev Viktor Vasil'Evich	RU2425052C1
METHOD OF PRODUCING 3β-ACETOXY-17α-HYDROPEROXY-16α-METHYLPREGNANES FROM Δ-20-KETOSTEROIDS AND METHOD OF PRODUCING 3β-ACETOXY-17α-HYDROXY-16α-METHYLPREGNANES USING 3β-ACETOXY-17α-HYDROPEROXY-16α-METHYLPREGNANES	2009	Savinova Tat'Jana Stepanovna Lukashev Nikolaj Vadimovich Beletskaja Irina Petrovna	RU2418805C1

RU 2 677 332 C1

Authors

Savinova Tatyana Stepanovna

Savinova Olga Sergeevna

Vavilova Ekaterina Aleksandrovna

Vasina Darya Vladimirovna

Tyazhelova Tatyana Vladimirovna

Fedorova Tatyana Vasilevna

Chulkin Andrej Mikhajlovich

Solev Pavel Nikolaevich

Dates

2019-01-16—Published

2017-11-17—Filed