BIOCATALYST FOR PRODUCING 11α-ACETOXYPROGESTERONE Russian patent published in 2019 - IPC C12P33/10 C12N1/14 A61K31/57 

FIELD: biotechnology.

SUBSTANCE: invention refers to biotechnology and concerns strains of microorganisms able to perform biotransformation of progesterone. Described is a new ability of known micellial fungi strains, specifically, a strain of wild type Aspergillus nidulans F-1069 (synonym FGSC A4; ATCC 3863, 12996, 26451; CBS 112.46; NRRL 194) and its auxotroph Aspergillus nidulans 031 (argB^-; pyrG^-), transform a steroid substrate: not only convert progesterone into 11α-hydroxyprogesterone, but also acetylated formed 11α-hydroxyprogesterone. Said ability is confirmed both by physical and chemical analysis methods of structure of organic compounds (mass spectrometry of high resolution, NMR-spectroscopy ¹H, ¹³C and 2D NMR) and counter chemical synthesis.

EFFECT: total selectivity 11α-hydroxylation reaches 99 %, and content of 11α-acetoxyprogesterone in the products mixture exceeds 47 %.

1 cl, 21 dwg, 2 tbl, 4 ex