FIELD: measurement; testing.
SUBSTANCE: group of inventions relates to detection of target marker in a biological sample. Kit for detecting a target marker in a biological sample in combination with a molecule, binding to the target marker, capable of specific binding to the target marker includes: first binding agent comprising a first binding molecule capable of directly or indirectly specifically binding to the molecule binding to the target marker and the labelling substance; linker molecule capable of specific binding to the first binding agent; and a second binding agent comprising a second binding molecule capable of specific binding to said linker molecule and a target substance. Wherein the labelling substance in the first binding agent and the labelling substance in the second binding agent may be the same or different; linker molecule is capable of specifically binding to the labelling substance in the first binding agent and the labelling substance in the second binding agent; first binding agent is a structure in which the first binding molecule and the labelling substance are connected to each other directly or indirectly by means of a carrier, which is a natural or synthetic polymer; second binding agent is a structure in which the second binding molecule and the labelling substance are connected to each other directly or indirectly by means of a carrier which is a natural or synthetic polymer. Method for detecting a target marker in a biological sample and the use of a kit for detecting a target marker in a biological sample are also disclosed.
EFFECT: group of inventions provides high detection sensitivity of the target marker.
11 cl, 11 dwg, 7 tbl
