SINGLE TARGET IMMUNOASSAY Russian patent published in 2015 - IPC G01N33/543 

FIELD: medicine.

SUBSTANCE: group of inventions refers to medicine, namely to laboratory diagnostics, and is applicable to visualise a single specific target unit in a sample wherein the above target is immobilised. That is ensured by incubating the sample containing a population of specific target units with a binding agent containing an enzyme; the binding agent is able to bind directly to the single specific target unit; the incubation process is followed by forming a single discrete target site with a fraction target sub-population, wherein each single discrete target site represents a complex of one specific unit of the above fraction sub-population and the binding agent. The sample is incubated in aqueous solution containing a peroxide compound in an amount making from 0.1 mM to 5 mM, a first enzymatic substrate bound to the single target sites, and a second enzymatic substrate, wherein the first substrate is a water-soluble electron-rich compound, to form a water-insoluble polymer product. The second substrate represents a conjugated molecule with a detectable label, wherein the detectable label is specified in a group consisting of fluorescent, luminescent, radioactive or chromogenic substances or members of specific binding pairs to form discrete deposits of the second substrate. Visualising the single target sites.

EFFECT: a method for quantitative estimation of the immobilised target in the sample, a diagnostic technique, a method for prediction of a risk of disease development and assessment of the efficacy of therapeutic treatment in the patient are also presented.

63 cl, 3 dwg, 5 tbl, 10 ex