FIELD: chemistry.
SUBSTANCE: it provides a method for detecting free antigen biospecific antibodies in the sample, wherein the antigen to be determined can be specifically linked to the first binding site biospecific antibodies comprising the sample incubation step comprising free antigen and biospecific antibody to anti-idiotypic antibody which specifically binds to second binding bispecific antibody determinant different from the first binding determinant, wherein the anti-idiotype antibody bound to a solid phase.
EFFECT: group of inventions can effectively determine the presence, concentration of free antigen biospecific antibodies in the sample.
9 cl, 13 dwg, 9 ex
