FIELD: biotechnology.
SUBSTANCE: nutrient medium to restore the number of anaerobic bacteria after low-temperature storage contains casein hydrolyzate, bactopeptone, yeast extract, glucose, sodium chloride, potassium phosphate, tris(hydroxymethylaminomethane), cysteine-L hydrochloride, agar-agar, 4-methylresorcinol and distilled water at a predetermined ratio of components. 4-methylresorcine is sterilely added to the autoclaved nutrient medium prepared without it.
EFFECT: invention allows to increase the efficiency of recovery of the number of anaerobic cultures of bacteria after low-temperature storage at the first passage and to reduce the time of cultivation of the biomass of bacteria.
1 cl, 2 tbl, 2 ex
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