TWO-PHASE NUTRITIONAL MEDIUM FOR THIN LAYER CULTIVATION OF Helicobacter pylori AND METHOD OF ITS REALISATION Russian patent published in 2014 - IPC C12N1/20 

FIELD: chemistry.

SUBSTANCE: invention relates to field of microbiology and can be applied in medicine. Method includes preliminary inoculation of analysed material on Columbia agar with addition of 5% of sheep blood. Thermostatting is performed under microaerophilic conditions in CO₂ incubator at 37°C for 3-5 days at higher humidity. Sampling colonies and their re-inoculation on Petri dishes with chocolate agar are performed and thermostatting is carried out under microaerophilic conditions in CO₂ incubator at 37°C for 3-5 days at higher humidity. When thermostatting stage is completed Petri dishes with chocolate agar are poured on the top with liquid nutritional medium based on Schaedler broth, enriched with 10% cattle serum with their further thermostatting in CO₂ incubator at 37°C for 72 hours.

EFFECT: obtaining nutritional medium.

4 cl