METHOD OF PRESERVING BACTERIA IN FAECAL MICROBIOTA AND BACTERIAL CULTURES GROWN ON DENSE AGARIZED NUTRIENT OR DIFFERENTIAL MEDIA IN LOW TEMPERATURE USING COMPOSITE MEDIUM FOR FREEZING Russian patent published in 2020 - IPC C12N1/04 C12N1/20 

FIELD: biotechnology.

SUBSTANCE: invention relates to the biotechnology. Disclosed is a method of preserving bacteria in faecal microbiota and bacterial cultures recovered from faecal microbiota grown on dense agarized nutrient or differential media. Method comprises mixing 0.1 g of sample in cryogenic test tube in 2 ml in ratio of 1:2 with cryoprotective medium consisting of distilled water 500 ml, bacteriological dry European agar 2 g, NaCl 2.5 g, yeast extract 1 g, L-cysteine 0.2 mg, pancreatic hydrolyzate of casein 8.5 mg, dextrose 5 g with addition of 10 % glycerine, vortex stirring for 2–5 min. Cryotubes put in by the cover are placed into the corresponding storage. Thawing of cryotubes is performed immediately before the tests.

EFFECT: method ensures preservation of titre and viability of bacteria during storage in conditions of low temperatures.

5 cl, 3 dwg, 2 tbl, 3 ex