METHOD OF EARLY PREDICTION OF DEVELOPMENT OF NEPHRITIS IN INDUCED MODEL OF SYSTEMIC LUPUS ERYTHEMATOSUS AUTOIMMUNE DISEASE IN VIVO Russian patent published in 2019 - IPC G01N33/48 A61K31/739 G09B23/28 

FIELD: veterinary medicine.

SUBSTANCE: invention relates to a method for predicting systemic inflammatory conditions in an animal that can lead to the development of nephritis in an induced model of an autoimmune disease of systemic lupus erythematosus. Method of early prediction of nephritis development in an induced model of an autoimmune disease of systemic lupus erythematosus includes administering a dose of lipopolysaccharide to healthy animals, followed by a simultaneous assessment of the level of inflammation in the blood cells and the level of extracellular DNA in the blood plasma. Lipopolysaccharide is intraperitoneally administered to healthy hybrids (C57BL/6 × DBA2)F1 at a dose of 50 mcg/kg, then for 2, 4, 8, 11 and 24 hours from the moment of its administration in the dynamics, the index of the ratio of neutrophils to lymphocytes (N/L) and extracellular DNA is evaluated in the blood and 24 hours after the administration of lipopolysaccharide a systemic lupus erythematosus model is induced by intravenous injection of cells of spleen of female mouse of DBA/2 line, some mice that have the maximum N/L index increase for 4 hours relative to the 2nd hour and the maximum level of extracellular DNA – for 8 hours, are count as the group of SLE^nephritis+ and the risk of the development of nephritis in this group is determined.

EFFECT: invention provides an earlier and accurate prediction of the development of nephritis in an induced model of systemic lupus erythematosus in vivo.

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