BIOLOGICAL MICROCHIPS FLUORIMETRIC ANALYZER Russian patent published in 2019 - IPC G01N21/64 G01N33/48 

FIELD: instrument engineering.

SUBSTANCE: invention relates to the quantitative luminescent microscopy used in devices intended for recording interactions between biological molecules, marked with the dye, fluorescent in the spectrum visible or infrared region, and immobilized in the biological microchip cells molecular probes. Disclosed is the biological microchips fluorimetric analyzer, containing the excitation radiation laser sources, filters for the biochip cells fluorescence isolation, an optical system for the biochips image projection onto the detector photosensitive matrix, and the spectra suppression device, containing the fiber-optic harness and rotating mirror, installed between the radiation source and the biochip cells matrix. At that, the mirror is installed so, that when it rotates within one revolution, the distance from the fiber-optic harness end to the mirror surface is constantly changing, as the detector a CCD camera is used, and the analyzer is configured to record and subsequently measure the biochip images series on the CCD camera with different exposures.

EFFECT: invention allows to determine the fluorescent dyes relative amounts in the biochip cells with accuracy of up to 10 %, enables the spectra suppression arising due to the coherent light interference on the microchip substrate, and increases the fluorescence intensity measured values range.

1 cl, 7 dwg, 3 tbl