FIELD: biotechnology.
SUBSTANCE: invention refers to biotechnology, molecular biology and biochemistry. Disclosed is a method for sequencing nucleic acids. Method involves polymerisation-catalysed inclusion of nucleotides in the formed nucleic acid filament relative to the nucleic acid matrix, where the polymerase is attached to a charge sensor which detects nucleotide incorporation events. For unique identification of different nucleotides in matrix nucleic acid one or more types of non-natural nucleotide can be used, each of which provides unique signatures on charge sensor.
EFFECT: invention enables more efficient reading of long fragments of nucleic acids which can be used in various scientific and applied fields.
42 cl, 10 dwg
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Year |
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|
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2020 |
|
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