FIELD: biotechnology.
SUBSTANCE: device for detection of nucleotides is presented, containing a conducting channel and from one to five polymerase molecules attached to the conducting channel. The surface of the conducting channel is modified by a set of surface modifiers. At the same time, the conducting channel contains nano-thread, as well as a set of linkers attaching surface modifiers to a set of complexes of nickel-nitrilotriacetic acid. Each of the mentioned one or more polymerase molecules contains a polyhistidine mark, which is related to one or more complexes of nickel-nitrilotriacetic acid. Methods for detection of nucleotide embedding to a formed nucleotide chain are also proposed.
EFFECT: invention allows for detection of embedding of nucleotide containing a charge mark to formed polynucleotide by means of polymerase.
26 cl, 6 dwg, 1 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| READING SYSTEMS | 2020 |
|
RU2791444C2 |
| CHARGE LABELLED NUCLEOTIDES AND METHODS FOR APPLICATION THEREOF | 2019 |
|
RU2751359C2 |
| SENSOR SYSTEMS | 2019 |
|
RU2772924C1 |
| SENSOR AND SENSOR SYSTEM FOR NUCLEIC ACID SEQUENCING | 2019 |
|
RU2747795C1 |
| METHOD OF DETECTING DNA POLYMERISATION REACTION RESULT AND A DEVICE FOR IMPLEMENTATION THEREOF | 2019 |
|
RU2724507C1 |
| FIELD DEVICE AND NUCLEIC ACID SEQUENCING METHODS | 2016 |
|
RU2685953C1 |
| LABELED NUCLEOTIDES AND THEIR USE | 2019 |
|
RU2782056C2 |
| METHOD FOR TAGLESS SINGLE-MOLECULAR DNA SEQUENCING AND A DEVICE FOR ITS IMPLEMENTATION BASED ON A CHARGE-SENSITIVE TRANSDUCER | 2021 |
|
RU2780050C1 |
| NUCLEOSIDES AND NUCLEOTIDES WITH 3'-HYDROXY BLOCKING GROUPS AND THEIR USE IN METHODS OF SEQUENCING POLYNUCLEOTIDES | 2019 |
|
RU2818762C2 |
| GENERALIZED STOCHASTIC SEQUESTRATION OF SUPERRESOLUTION | 2019 |
|
RU2739571C1 |
