FIELD: biotechnology.
SUBSTANCE: device for detection of nucleotides is presented, containing a conducting channel and from one to five polymerase molecules attached to the conducting channel. The surface of the conducting channel is modified by a set of surface modifiers. At the same time, the conducting channel contains nano-thread, as well as a set of linkers attaching surface modifiers to a set of complexes of nickel-nitrilotriacetic acid. Each of the mentioned one or more polymerase molecules contains a polyhistidine mark, which is related to one or more complexes of nickel-nitrilotriacetic acid. Methods for detection of nucleotide embedding to a formed nucleotide chain are also proposed.
EFFECT: invention allows for detection of embedding of nucleotide containing a charge mark to formed polynucleotide by means of polymerase.
26 cl, 6 dwg, 1 tbl, 1 ex
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