FIELD: veterinary medicine.
SUBSTANCE: invention refers to molecular biology and veterinary science. First, high-active and high-specific DNA-containing T. evansi and T. equiperdum antigens are obtained from the blood of sick horses or blood of mice and rabbits which are three times administered with a trypanosomal antigen with an adjuvant (aluminum hydroxide) and which are exsanguinated on 25–30th day lyophilized, and then used in polymerase chain reaction with primers to the RoTat1.2 VSG gene, subunit gene 5 of NADH dehydrogenase (nad5) with subsequent electrophoretic determination of the size of the amplified fragment of the nucleotide sequence. If there is 410 bp fragment on the electrophoregram, presence of T. evansi DNA DNA-containing antigens is diagnosed; and in presence of 724 bp fragment, presence of T. equiperdum DNA DNA-containing antigens in the samples, respectively. Method enables reliable differentiation of trypanosomes of T. evansi and T. equiperdum samples.
EFFECT: invention is intended for differential diagnosis of breeding sickness and horse surra.
1 cl, 6 dwg
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