DIAGNOSTIC TECHNIQUE FOR CATTLE LEUKAEMIA BY POLYMERASE CHAIN REACTION Russian patent published in 2012 - IPC C12Q1/68 

FIELD: medicine.

SUBSTANCE: upstream and downstream oligonucleotide primers are used for separating a pol gene fragment of cattle leukaemia provirus. It is followed by electrophoretic measurement of an amplified fragment of the nucleotide sequence. The oligonucleotides of the following structure P_F2 are used as primers: 5'-TGA ACG GAC AAA TGG ACT GCT C-3'; PR2: 5'-CCG ACA GAG AGC GAG GAG AG-3', wherein there are found no self-complementary sites inside each primer and between upstream and downstream ones; firing temperature makes 66°C for both oligonucleotides; GC composition is 50% for P_F2 and 65% for P_R2, and which flank a region of the conservative pol gene of cattle leukaemia virus of size 438 base pairs.

EFFECT: technique is reliable, high-sensitive and high-specific method which can be used to detect an antiviral CLV DNA fragment in a biological material.

3 dwg, 3 tbl, 3 ex