FIELD: medicine.
SUBSTANCE: proposed group of inventions relates to medicine. Disclosed are a method and a kit for detecting Q61R mutation in a NRAS protein in a human tumor tissue sample. Method involves obtaining a sample of biological material taken from a patient, recovering DNA from said sample and carrying out PCR using a kit for detecting Q61R mutation in a NRAS protein.
EFFECT: presented group of inventions enables higher specificity and sensitivity of determining the presence of a mutant allele at the Q61R point of the NRAS gene, as well as reducing the probability of process errors during laboratory manipulations.
3 cl, 1 dwg, 1 tbl, 1 ex
Title |
Year |
Author |
Number |
METHOD FOR ANALYSIS OF SOMATIC MUTATIONS IN BRAF, NRAS AND KIT GENES USING LNA-BLOCKING MULTIPLEX PCR AND SUBSEQUENT HYBRIDIZATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) |
2017 |
- Emelyanova Marina Aleksandrovna
- Zasedatelev Aleksandr Sergeevich
- Nasedkina Tatyana Vasilevna
|
RU2674338C1 |
QUANTITATIVE METHOD FOR DETERMINING THE EXPRESSION OF GNAO1 ALLELES OF A HEALTHY FORM AND WITH c.607 G>A MUTATION |
2021 |
- Lunev Evgenii Andreevich
- Savchenko Irina Mikhailovna
- Bardina Mariana Vladimirovna
- Polikarpova Anna Vadimovna
|
RU2777663C1 |
USE OF SPECIFIC MARKERS OF BROWN AVERAGE FIBRE 3 (BROWN MIDRIB-3) IN CORN FOR DISPERSAL SIGNS |
2011 |
- Vej Chen
- Van Opdorp Natan
- Channabasavaradkhya Chandra-Shekara
- Kumpatla Siva P.
|
RU2593958C2 |
METHOD FOR DETERMINING HELICOBACTER PYLORI |
2017 |
|
RU2744190C1 |
METHOD, SEQUENCES, COMPOSITIONS AND KIT FOR DETECTING CHANGES IN PROMOTER OF hTERT GENE |
2016 |
- Soares Dias Ferreira, Ana Paula
- Marques Prazeres, Hugo Joao
- Alves Salgado, Catarina Miguel
- Monteiro Batista, Rui Pedro
- Rico De Oliveira Vinagre, Joao Pedro
|
RU2720257C2 |
METHODS AND SETS RELATED TO CAPTURE OF CA-IX-POSITIVE EXOSOMES |
2018 |
- Chiesi, Antonio
- Zarovni, Natasa
- Zocco, Davide
|
RU2770592C2 |
METHOD FOR FAD3 GENE ZYGOSITY DETERMINATION IN CANOLA |
2012 |
- Ubayasena Lasanta Chandana
- Elert Zoe
- Channabasavaradkhya Chandra Shekara A.
- Gupta Mandzhu
|
RU2630998C2 |
METHOD FOR ANALYSIS OF SOMATIC MUTATIONS IN GNAQ AND GNA11 GENES USING LNA-BLOCKING MULTIPLEX PCR AND SUBSEQUENT HYBRIDIZATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) |
2017 |
- Emelyanova Marina Aleksandrovna
- Zasedatelev Aleksandr Sergeevich
- Nasedkina Tatyana Vasilevna
|
RU2674687C1 |
METHOD OF DETECTING MUTATIONS IN COMPLEX DNA MIXTURES |
2014 |
|
RU2613489C2 |
METHOD FOR ANALYSIS OF SOMATIC MUTATIONS IN PI3K GENE USING LNA-BLOCKING MULTIPLEX PCR AND SUBSEQUENT HYBRIDISATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) |
2013 |
- Barskij Viktor Evgen'Evich
- Nasedkina Tat'Jana Vasil'Evna
- Emel'Janova Marina Aleksandrovna
- Abramov Ivan Sergeevich
- Pan'Kov Sergej Vasil'Evich
|
RU2549682C1 |