FIELD: biotechnology.
SUBSTANCE: invention refers to biotechnology, molecular biology and biochemistry. Disclosed is a method for changing a DNA target in a Cas-expressing cell expressing a complex of colocalization with an RNA guide complementary to a DNA target which cleaves the DNA target in a site-specific manner, followed by insertion of the donor nucleic acid into the target DNA.
EFFECT: invention makes it possible to perform genome editing with high efficiency and can be used in medicine.
25 cl, 20 dwg, 20 ex, 6 tbl
| Title | Year | Author | Number |
|---|---|---|---|
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|
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| GENOME ENGINEERING | 2021 |
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| RNA-GUIDED HUMAN GENOME ENGINEERING | 2013 |
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| GUIDED RNA TRANSCRIPTION REGULATION | 2014 |
|
RU2690935C2 |
| RNA-GUIDED TRANSCRIPTION REGULATION | 2014 |
|
RU2756865C2 |
| GENE EDITING USING MODIFIED CLOSED-END DNA (ceDNA) | 2018 |
|
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| METHODS AND COMPOSITIONS FOR NUCLEASE-MEDIATED TARGETING OF TRANSGENES | 2013 |
|
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| PCDEXO-GS-SSB GENETIC CONSTRUCTS CONTAINING SEQUENCES ENCODING CHIMERIC SSB PROTEINS AND EXONUCLEASES OF THE BACTERIOPHAGE HOMOLOGOUS RECOMBINATION APPARATUS, COMBINED VIA A GLYCINE-SERINE POLYLINKER | 2020 |
|
RU2763747C1 |
| MULTIPLEX RNA-GUIDED GENOME ENGINEERING | 2014 |
|
RU2706562C2 |
