FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, namely, to production of a recombinant SSB-GS-EXO protein construct. Proposed is a method for producing a recombinant SSB-GS-EXO protein construct, involving transformation of competent cells and expression of genetic constructs therein based on a plasmid DNA, wherein 5’-3'Flap exonuclease (EXO) and SSB protein are combined by genetic engineering through a glycine-serine spacer represented by the sequence SEQ ID NO: 1.
EFFECT: invention ensures cloning of DNA molecules with an increased frequency of homologous recombination between nucleic acids in the cell.
1 cl, 3 dwg, 1 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| FAD2 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS | 2013 |
|
RU2656159C2 |
| GENOME EDITING TOOL BASED ON LIGD PROTEIN FROM THE BACTERIUM PSEUDOMONAS PUTIDA AND CAS9 COMPLEX | 2022 |
|
RU2797049C1 |
| FAD3 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS | 2013 |
|
RU2665811C2 |
| GENE EDITING USING MODIFIED CLOSED-END DNA (ceDNA) | 2018 |
|
RU2811724C2 |
| GENOMIC ENGINEERING | 2014 |
|
RU2688462C2 |
| CONSTRUCTED BY ENGINEERING TECHNIQUES PLATFORM (ETIP) FOR TRANSGENE EMBEDDING FOR GENETIC TARGETING AND TRAIT STACKING | 2013 |
|
RU2666916C2 |
| METHODS AND COMPOSITIONS FOR NUCLEASE-MEDIATED TARGETING OF TRANSGENES | 2013 |
|
RU2650819C2 |
| RECOMBINANT PLASMID DNAS PX458D10A_MYC, PROVIDING MYC GENE KNOCKOUT IN HUMAN CELLS, METHOD OF PRODUCING HUMAN CELL LINE WITH MYC GENE KNOCKOUT AND HUMAN BREAST CANCER CELL LINE MDA-MB231_MYC WITH SUPPRESSED MYC GENE EXPRESSION | 2022 |
|
RU2818348C1 |
| FAD2 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS | 2013 |
|
RU2656158C2 |
| IMPROVED EUCARYOTIC CELLS FOR PROTEIN PRODUCTION AND METHODS FOR THEIR PRODUCTION | 2016 |
|
RU2752529C2 |
