FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, in particular to guiding RNA containing a spacer complementary to this target nucleic acid, and a frame attached to the specified spacer, where the frame binds Cas protein of type II CRISPR system, where guiding RNA contains from about 100 to about 250 nucleotides, as well as to a genome edition system and a cell containing it. Nucleic acid encoding the specified guiding RNA, as well as a structure and a virus containing it, are also disclosed.
EFFECT: invention is effective in Cas-based RNA-guided genome edition of an eucaryotic cell.
17 cl, 15 dwg, 18 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RNA-GUIDED ENGINEERING OF HUMAN GENOME | 2013 |
|
RU2699523C2 |
| GENOMIC ENGINEERING | 2014 |
|
RU2764757C2 |
| GENOME ENGINEERING | 2021 |
|
RU2812848C2 |
| RNA-GUIDED TRANSCRIPTION REGULATION | 2014 |
|
RU2756865C2 |
| GENOMIC ENGINEERING | 2014 |
|
RU2688462C2 |
| METHODS AND COMPOSITIONS FOR TARGETED GENETIC MODIFICATION USING PAIRED GUIDE RNAS | 2015 |
|
RU2734770C2 |
| GUIDED RNA TRANSCRIPTION REGULATION | 2014 |
|
RU2690935C2 |
| ARTIFICIAL GENOME MODIFICATION FOR GENE EXPRESSION REGULATION | 2018 |
|
RU2767201C2 |
| RNA-CONDUCTOR MOLECULE FOR GENOMIC EDITING OF PROMOTER REGION OF VRN-A1 GENE OF MONOCOTYLEDONOUS CEREALS USING CRISPR/CAS9 SYSTEM | 2020 |
|
RU2762831C1 |
| CRISPR-CAS SYSTEMS AND METHODS FOR CHANGING THE EXPRESSION OF GENE PRODUCTS | 2013 |
|
RU2796273C2 |
