FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, in particular to methods for changing DNA of target nucleic acid in a cell, which provide for the introduction to the cell of the first alien nucleic acid encoding two or more RNA, wherein each RNA is complementary to a site adjacent to DNA of target nucleic acid, and introduction to the cell of the second alien nucleic acid encoding at least one Cas9 protein-nicase with one inactive nuclease domain or DNA-binding protein-nicase from CRISPR system of II type. Cells for RNA-guided Cas-based genome editing are also disclosed.
EFFECT: invention allows for effective changing DNA of target nucleic acid in a cell.
40 cl, 21 dwg, 2 tbl, 17 ex
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