GUIDED RNA TRANSCRIPTION REGULATION Russian patent published in 2019 - IPC A61K48/00 C12N15/00 C07H21/02 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology. Methods are presented for modulating expression of nucleon velocity in cells, including introduction into cells of first foreign nucleic acid coding one or more RNA, complementary to DNA, wherein the DNA comprises the target nucleic acid, introducing into the cells a second foreign nucleic acid encoding a non-nucleated Cas9 protein which binds to DNA and is directed by one or more RNAs, introducing into cells a third foreign nucleic acid coding a transcriptional control protein or domain, wherein one or more RNA, non-nucleated Cas9 protein and transcriptional control protein or domain are expressed, wherein one or more RNA, the non-nucleated Cas9 protein and the transcriptional control protein or domain are co-localized on DNA, and the transcriptional control protein or domain regulates the expression of the target nucleic acid.

EFFECT: disclosed is guided RNA transcription regulation.

29 cl, 21 dwg, 2 tbl, 17 ex