METHOD FOR DETERMINING A PERSONALIZED CRYOPROTECTOR BY LEUKOCYTE ACID PHOSPHATASE OF PRESERVED BLOOD Russian patent published in 2019 - IPC G01N33/48 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to laboratory diagnostics. Method for determining a personal cryoprotective agent by leukocyte acid phosphatase (LAP) of preserved blood involves exuding a portion of fresh autoblood before the beginning of the component transfusion therapy, stabilized with sodium citrate solution, divided into test tubes into equal parts, in control sample (CS) cryoprotective additives are excluded, in test samples (TS) is added by one equal dose of tested cryoprotectors, mixed at plus 37 °C for 4 hours, droplets of prepared biological fluids from TS and CS are applied in volume of 4 mcl on the slide, 2-3 smears are made, dried in air, fixed in 10 % alcohol-formalin mixture, leukocytes are stained for acid phosphatase according to Bereston's azocoupling method in Yu.F. Rudens, I.M. Buikis count is microscopic in transmitted light; LAP content is calculated in segmented nucleated (s/n) neutrophils and lymphocytes with calculation of average cytochemical coefficient (CCC); with coincidence of values of CCC parameters of TS and CCC CS, tested cryoprotector is evaluated as optimal for a specific patient and suitable for use; at CCC TS values, other than CCC CS, conclusion is made as unfit to cryoprotector.

EFFECT: disclosed is a method of determining a personalized cryoprotector.

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