METHOD FOR DETERMINING AMOUNT OF COLLAGEN IN TISSUE Russian patent published in 2019 - IPC G01N33/48 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, histology and can be used for quantitative determination of tissue collagen. Method for determining the amount of collagen in tissue involves multiple homogenisation of material obtained by freezing, lyophilic drying, milling a pre-weighed piece of tissue, followed by resuspending the material using a dispenser and centrifuging, then prepared material is frozen at -80 °C and lyophilic drying, obtained water-free material is weighed, material weight m₁ is determined, material is dissolved in 900 mcl of a buffer solution with pH 7.0 and added with 100 mcl of collalisin solution, which is prepared beforehand by dissolving the contents of one ampoule of collalysin, containing collagenase in the form of lyophilizate in amount of 1.000 KE, in 2 ml of a buffer solution with pH 7.0, test tube with contents is stirred for 2 hours, three times a cycle of homogenisation, centrifugation at 13,400 rpm and resuspension of material, material is frozen at -80 °C, is lyophilically dried and weighed, material weight m₂ is determined, by mass difference m₁-m₂ weight of collagen is determined.

EFFECT: disclosed is a method for determining the amount of collagen in tissue.

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