METHOD FOR EXTRACTING ISOLATED CELLS FROM THE JAW Russian patent published in 2022 - IPC G01N33/48 G01N1/28 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely, to histology, dentistry and cytology. Pre-crushed jaw samples fixed in formalin are treated with 50% potassium hydroxide at 20°C. The material is then homogenised with a jet of water from a Pasteur pipette, followed by suspending and executing at least five cycles of homogenisation and centrifugation of the material at 1,500 rpm for 5 minutes until the pH of the supernatant liquid reaches 6.0. The material is transferred onto glass for further study. Prior to the treatment with an alkaline solution, the jaw herein is exposed to the SoftiDec decalcifying solution based on ethylenediaminetetraacetate (EDTA), pre-diluted in 2 with distilled water, imparting a shape to the jaws that allows grinding, treating with an alkali solution, and extracting isolated cells for further study.

EFFECT: method provides a possibility of expanding the possibilities of extracting isolated jaw cells for further study of the morphology thereof.

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