METHOD FOR CALCULATING DEGREE OF ALVEOLAR MACROPHAGE SURFACE DAMAGE FROM ALUMINUM OXIDE PARTICLES Russian patent published in 2019 - IPC G01N33/483 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to laboratory diagnostics, and enables calculating degree of alveolar macrophage surface damage caused by alumina particles. For this purpose, the experimental individual undergoes a single intratracheal exposure with a suspension of aluminum oxide particles. That is followed by single airway irrigation with sterile isotonic physiological solution. Cytological smears are obtained from the obtained washing water. Alveolar macrophages are recovered from cells of the above smears and three-dimensional models of alveolar macrophage surface are constructed for each separately. Digital photographic survey of each individual cell is carried out at different focal distances. Obtained images are converted into black and white eight-bit images and the background image is subtracted, on the basis of which three-dimensional models of the surface of alveolar macrophages are constructed for each separately. Using the obtained three-dimensional model of the surface of the alveolar macrophage, the following parameters are determined: diameter of injuries and number of damages per one cell. Then average values for each of the above parameters are determined. Total surface area of alveolar macrophage is determined. Degree of damage of the surface of each individual cell of the alveolar macrophage is calculated. Then average value of this degree is summed up among analyzed cells according to the equation. Relation of degree of alveolar macrophage damage from action of particles of aluminum oxide and total specific surface area of particles is considered reliable at value of total specific surface area of particles of aluminum oxide more than 0.05 m².

EFFECT: invention allows assessing the alveolar macrophage damage depending on the quantitative parameter – total specific surface area of aluminum oxide particles, which caused this pathological change precisely in this degree.

4 cl, 3 dwg, 2 tbl, 1 ex