FIELD: biotechnology.
SUBSTANCE: described is a method for detecting the coronavirus infection pathogen genome in cattle. Method includes isolation of RNA from biological material in form of extract of excrements or fragments of abdominal cavity by sorption method, synthesis of cDNA on RNA matrix by single-step staging with addition of internal and positive control samples of multiplex reverse transcription reaction and polymerase chain reaction - with 45 cycles of amplification with real-time detection using oligonucleotide primers specific for the virus genome region of fluorescent-labelled probe. Fragment of the coronavirus infection agent genome has the following nucleotide sequences: BCoVF 5'-GATCAAATTGCTAGT-3' - forward primer; BCoVR 5'-CAGTCTGCTTAGTTA-3' - reverse primer; BCoVP 5'-FAM-GGATGCCACTAAGCCA-3'-BHQ1 is a probe.
EFFECT: invention extends the range of methods for diagnosing coronavirus infection in cattle.
1 cl, 4 tbl, 1 ex
Title | Year | Author | Number |
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TEST SYSTEM FOR DETECTING CORONAVIRUS INFECTION PATHOGEN GENOME IN CATTLE BY MEANS OF MULTIPLEX POLYMERASE CHAIN REACTION WITH FLUORESCENT DETECTION IN REAL TIME | 2018 |
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METHOD FOR DETECTING GENOME OF ROTAVIRUS INFECTION AGENT IN FARM ANIMALS | 2018 |
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RU2689718C1 |
TEST SYSTEM FOR GENOME DETECTION OF A CORONAVIRUS INFECTION AGENT OF NEW TYPE (NCOV19) IN PRIMATES | 2020 |
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RU2741887C1 |
TEST SYSTEM FOR GENOME DETECTION OF ROTAVIRUS AGENT OF TYPE A IN AGRICULTURAL ANIMALS BY MEANS OF MULTIPLEX POLYMERASE CHAIN REACTION WITH FLUORESCENT DETECTION IN REAL TIME | 2018 |
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RU2694501C1 |
TEST SYSTEM FOR DETECTING RNA OF AGENT OF ARTERITIS VIRUS IN HORSES | 2018 |
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RU2698662C1 |
METHOD FOR DETECTING RNA OF AN ARTERITIS VIRUS AGENT IN HORSES | 2018 |
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RU2700481C1 |
TEST SYSTEM FOR DETECTION OF THE VIRUS GENOME OF PARAINFLUENZA 3 TYPES AT CATTLE WITH A MULTIPLEX POLYMERASE CHAIN REACTION WITH FLUORESCENT DETECTION IN REAL TIME MODE | 2018 |
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RU2681473C1 |
METHOD FOR DETECTING GENOME OF PARAINFLUENZA VIRUS OF TYPE 3 IN BOVINE ANIMALS | 2018 |
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RU2696069C2 |
METHOD FOR DETECTING AND GENOTYPING RNA OF PORCINE REPRODUCTIVE-RESPIRATORY SYNDROME VIRUS | 2018 |
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RU2703394C1 |
Authors
Dates
2019-07-16—Published
2018-01-16—Filed