THE METHOD OF PRODUCTION OF INTERLEUKIN-36 RECEPTOR ANTAGONIST WITH CLEAVED N-TERMINAL METHIONINE, ESCHERICHIA COLI BL21[DE3] STRAIN PRODUCING INTERLEUKIN-36 RECEPTOR ANTAGONIST WITH CLEAVED N-TERMINAL METHIONINE (VARIANTS), METHOD OF THEIR CONSTRUCTION, PLASMID VECTORS pBAD15A AND pET15A AND DNA SEQUENCE FOR THEIR CONSTRUCTION Russian patent published in 2018 - IPC C12N1/21 C12N15/11 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology, in particular to a technology of Interleukin-36 receptor antagonist production, and constitutes the plasmid expression vector pBAD15A for production of human Interleukin-36 receptor antagonist (IL-36Ra) with cleaved N-terminal methionine, the map of said vector is shown on Fig. 2, the plasmid expression vector pET15A for production of human Interleukin-36 receptor antagonist (IL-36Ra) with cleaved N-terminal methionine, the map of said vector is shown on Fig. 3, as well as the strain producing the human Interleukin-36 receptor antagonist (IL-36Ra) with cleaved N-terminal methionine.

EFFECT: inventions allow to obtain more active IL-36Ra with a higher yield.

5 cl, 12 dwg, 7 ex, 4 tbl