Show metadata Hide metadata

(19)

(11)

2 760 585

(13)

(51)

IPC

C12N1/21(2006-01-01)

C12N15/20(2006-01-01)

C12N15/70(2006-01-01)

C07K14/555(2006-01-01)

C12R1/19(2006-01-01)

(21) (22)

Application

2020143510, 2020-12-28

(24)

Start date

2020-12-28

(22)

Actual filing date

2020-12-28

(45)

Published

2021-11-29

(72)

Inventor

Vasin Andrej VladimirovichGyulikhandanova Nataliya EvgenevnaLozhkov Aleksej AleksandrovichTaraskin Aleksandr SergeevichElshin Nikita DmitrievichZabrodskaya Yana AleksandrovnaKlotchenko Sergej AnatolevichPlotnikova Marina AleksandrovnaShaldzhyan Aram Arutyunovich

(73)

Holder

Federalnoe Gosudarstvennoe Avtonomnoe Obrazovatelnoe Uchrezhdenie Vysshego Obrazovaniya Politekhnicheskij Universitet Petra Velikogo" Vo

STRAIN OF ESCHERICHIA COLI BACTERIA - PRODUCER OF IL-29 RECOMBINANT PROTEIN Russian patent published in 2021 - IPC C12N1/21 C12N15/20 C12N15/70 C07K14/555 C12R1/19

Abstract RU 2760585 C1

FIELD: biotechnology.

SUBSTANCE: proposed is a recombinant plasmid DNA pet302-NT-His-hIFN-λ₁ for expression of the recombinant interferon protein hIFN-λ₁ with the sequence SEQ ID NO:3 in E. coli cells. Also proposed is a strain of Escherichia coli BL21[DE3]pET302-hIFNL1 - producer of the recombinant interferon protein hIFN-λ₁ with the sequence SEQ ID NO:3. Said strain is therein produced by transforming cells of the BL21DE3 E. coli strain by the recombinant plasmid DNA pet302-NT-His-hIFN-λ₁.

EFFECT: produced lambda interferon is stable in storage and can be used as a therapeutic agent.

2 cl, 4 dwg, 8 ex

Similar patents RU2760585C1

Title	Year	Author	Number
ESCHERICHIA COLI BL21(DE3)PLYSS/PET15B-HISCPF1 STRAIN: PRODUCER OF RNA-GUIDED ENDONUCLEASE CRISPR/CPF1	2021	Vasikhovskaya Valeriya Aleksandrovna Romanenko Margarita Vladimirovna Netesov Sergej Viktorovich	RU2774120C1
RECOMBINANT PLASMID DNA ENCODING CHIMERIC INTERFERON ALPHA2b, RECOMBINANT STRAIN OF YEAST P. PASTORIS X33 - PRODUCER OF CHIMERIC INTERFERON ALPHA2b, AND METHOD FOR PRODUCING THIS PROTEIN	2020	Beklemishev Anatolij Borisovich Pykhtina Mariya Borisovna	RU2764787C1
FRAGMENT OF DNA, CODING SYNTHESIS OF GLYCOPROTEIN OF RABIES G VIRUS, RECOMBINANT PLASMIDAL DNA PVG18-1, CODING GLYCOPROTEIN OF RABIES G VIRUS, ISOLATE OF BACTERIUM EXCHERICHIA COLI IS PRODUCENT OF GLYCOPROTEIN OF RABIES G VIRUS	1991	Grabko Vladimir Ivanovich	RU2008355C1
RECOMBINANT FUSED PROTEIN	2019	Maksimyak Sergej Petrovich Al-Shekhadat Ruslan Ismailovich	RU2732795C1
METHOD FOR PRODUCING RECOMBINANT PROTEIN OF SERRATIA MARCESCENS BACTERIA NUCLEASE FOR HYDROLYSIS OF NUCLEIC ACIDS	2021	Grunina Tatyana Mikhajlovna Lyashchuk Aleksandr Mikhajlovich Galushkina Zoya Mikhajlovna Lavrova Natalya Vitalevna Soboleva Lyubov Aleksandrovna Nikitin Kirill Evgenevich Lunin Vladimir Glebovich	RU2773954C1
NUCLEOTIDE SEQUENCE ENCODING β-N-ACETYLHEXOZAAMINIDASE AGGREGATIBACTER ACTINOMYCETEMCOMITANS AND GENETIC STRUCTURES CONTAINING IT	2021	Al-Shekhadat Ruslan Ismailovich	RU2789544C1
RECOMBINANT SERRATIA MARCESCENS NUCLEASE PROTEIN FOR HYDROLYSIS OF NUCLEIC ACIDS	2022	Grunina Tatyana Mikhajlovna Lyashchuk Aleksandr Mikhajlovich Galushkina Zoya Mikhajlovna Lavrova Natalya Vitalevna Soboleva Lyubov Aleksandrovna Nikitin Kirill Evgenevich Lunin Vladimir Glebovich	RU2787186C1
CODING THE HYBRID PROTEIN RECOMBINANT PLASMID DNA PERIG-PGS, ABLE TO AUTOCATALIC SPLITTING WITH FORMATION OF THE PIGASTINE ANTI-ANGIOGENIC PEPTIDE - DERIVATIVE OF THE HUMAN PIGMENTAL EPITHELY GROWTH FACTOR FRAGMENT [44-77], STRAIN ESCHERICHIA COLI BL21(DE3)/PERIG-PGS - PRODUCER OF THE SAID PROTEIN, AND THE RECOMBINANT ANTI-ANGIOGENIC PEPTIDE PRODUCTION METHOD	2017	Esipov Roman Stanislavovich Kostromina Mariya Andreevna Bejrakhova Kseniya Andreevna Makarov Dmitrij Aleksandrovich Miroshnikov Anatolij Ivanovich	RU2664199C1
RECOMBINANT PLASMID DNA pHIG05, CODING HYBRID PROTEIN WITH HUMAN PROINSULIN Glargine, CELL Escherichia coli, TRANSFORMED BY RECOMBINANT PLASMID DNA PHIG05, AND STRAIN OF BACTERIA Escherichia coli JM109/pHIG05-PRODUCENT OF HYBRID PROTEIN WITH HUMAN PROINSULIN Glargine	2013	Shmatchenko Vadim Vasil'Evich Sadgjan Armen Sergeevich	RU2515115C1
RECOMBINANT PLASMID DNA pET19b-SurvOL, WHICH PROVIDES SYNTHESIS OF A HYBRID PROTEIN SURVIVIN-OBELIN (SurvOL) AND A HYBRID PROTEIN BOUND BY ANTI-SURVIVIN ANTIBODIES AND POSSESSING BIOLUMINESCENT ACTIVITY	2021	Bashmakova Evgeniia Evgenevna Panamarev Nikita Sergeevich Frank Liudmila Alekseevna	RU2770490C1

RU 2 760 585 C1

Authors

Vasin Andrej Vladimirovich

Gyulikhandanova Nataliya Evgenevna

Lozhkov Aleksej Aleksandrovich

Taraskin Aleksandr Sergeevich

Elshin Nikita Dmitrievich

Zabrodskaya Yana Aleksandrovna

Klotchenko Sergej Anatolevich

Plotnikova Marina Aleksandrovna

Shaldzhyan Aram Arutyunovich

Dates

2021-11-29—Published

2020-12-28—Filed