GENETIC CONSTRUCT FOR INDUCING PROLIFERATION OF PERIPHERAL MONOCYTES IN VITRO Russian patent published in 2019 - IPC C12N15/63 C12N15/83 C12N15/12 C12N5/16 

FIELD: biotechnology.

SUBSTANCE: disclosed is a genetic construct for inducing the proliferation of peripheral monocytes and dendrite cells in vitro derived from human blood, comprising sequences coding transcription factors c-Mus and BMI1, as well as their binding self-splitting peptide sequence of Thosea asigna T2A virus, providing polycistronic expression of transcription factors under the control of one EF1alpha promoter. Said construct is derived from expression plasmid lentiviral vector pLEF1a-tagRFP, including a number of regulatory sequences: 5' and 3' LTR long end repeats, Rev dependent element (RRE or Rev-response element), Env is a regulatory sequence providing packaging of a viral genome into a viral particle structure with formation of a virion, cPRT - central polypurine path, a post-transcriptional regulatory element WPRE, an EF1alpha promoter.

EFFECT: invention provides effective constitutive expression of transcription factors s-Mus and BMI1 when introducing said construct into peripheral monocytes and dendritic cells.

1 cl, 3 dwg, 3 ex