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2 627 181

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IPC

C12N15/867(2006-01-01)

(21) (22)

Application

2015156511, 2015-12-29

(24)

Start date

2015-12-29

(22)

Actual filing date

2015-12-29

(45)

Published

2017-08-03

(72)

Inventor

Chumakov Stepan PetrovichRatnikova Natalya MikhajlovnaKravchenko Yuliya EvgenevnaLezhnin Yurij NikolaevichKovriga Irina EvgenevnaFrolova Elena Ivanovna

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Uchrezhdenie Nauki Institut Bioorganicheskoj Khimii Im. Akademikov M.M. Shemyakina I Yu.A. Ovchinnikova Rossijskoj Akademii Nauk Ran)

EXPRESSIONAL PLASMID LENTIVIRAL VECTOR FOR HETEROLOGICAL EXPRESSION OF RECOMBINANT HUMAN PROTEIN CD47 Russian patent published in 2017 - IPC C12N15/867

Abstract RU 2627181 C2

FIELD: biotechnology.

SUBSTANCE: invention relates to a plasmid lentiviral vector for heterologous expression of a recombinant human CD47 protein in mammalian cells. The vector contains a highly functional cytomegalovirus promoter (CMV), a CD47 receptor extracellular domain sequence, a TEV cysteine protease recognition sequence, a tagRFP fluorescent protein sequence acting as a selectable marker. All three sequences are in the same reading frame under the control of this promoter.

EFFECT: invention allows to obtain a highly productive and stable protein expression system for the further production of its purified form.

2 cl, 1 ex, 4 dwg

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RU 2 627 181 C2

Authors

Chumakov Stepan Petrovich

Ratnikova Natalya Mikhajlovna

Kravchenko Yuliya Evgenevna

Lezhnin Yurij Nikolaevich

Kovriga Irina Evgenevna

Frolova Elena Ivanovna

Dates

2017-08-03—Published

2015-12-29—Filed