FIELD: biotechnology.
SUBSTANCE: invention relates to a plasmid lentiviral vector for heterologous expression of a recombinant human CD47 protein in mammalian cells. The vector contains a highly functional cytomegalovirus promoter (CMV), a CD47 receptor extracellular domain sequence, a TEV cysteine protease recognition sequence, a tagRFP fluorescent protein sequence acting as a selectable marker. All three sequences are in the same reading frame under the control of this promoter.
EFFECT: invention allows to obtain a highly productive and stable protein expression system for the further production of its purified form.
2 cl, 1 ex, 4 dwg
| Title |
Year |
Author |
Number |
| EXPRESSION PLASMID LENTIVIRAL VECTOR FOR HETEROLOGICAL EXPRESSION OF HUMAN CD44 RECOMBINANT PROTEIN |
2015 |
- Chumakov Stepan Petrovich
- Ratnikova Natalya Mikhajlovna
- Lezhnin Yurij Nikolaevich
- Kravchenko Yuliya Evgenevna
- Kovriga Irina Evgenevna
- Frolova Elena Ivanovna
|
RU2646111C2 |
| AUXILIARY PLASMID LENTIVIRAL EXPRESSION VECTOR FOR OBTAINING HIGH TITERS OF Vpx-CONTAINING LENTIVIRAL PARTICLES, WHICH PROVIDES EFFECTIVE INFECTION OF HUMAN MONOCYTES AND DENDRITIC CELLS |
2017 |
- Chumakov Stepan Petrovich
- Kravchenko Yuliya Evgenevna
- Ivanova Anna Evgenevna
- Frolova Elena Ivanovna
|
RU2697781C2 |
| GENETIC CONSTRUCT FOR INDUCING PROLIFERATION OF PERIPHERAL MONOCYTES IN VITRO |
2017 |
- Chumakov Stepan Petrovich
- Kravchenko Yuliya Evgenevna
- Frolova Elena Ivanovna
|
RU2697797C2 |
| RECOMBINANT PLASMID DNA OF PTRX-TEVRS-PTH CODING A HYBRID PROTEIN CAPABLE OF PROTEOLYTIC SPLITTING TO FORM A FRAGMENT OF ENDOGENOUS HUMAN PARATHYROID HORMONE (1-34), AN ESCHERICHIA COLI BL21(DE3)/PTRX-TEVRS-PTH STRAIN - PRODUCER OF SAID PROTEIN AND A METHOD OF PRODUCING RECOMBINANT PTH (1-34) |
2019 |
- Myagkikh Igor Valentinovich
- Stepanenko Vasilij Nikolaevich
- Puchkov Ilya Aleksandrovich
- Novozhilov Nikolaj Mikhajlovich
- Makarov Dmitrij Aleksandrovich
- Sokolova Irina Vladimirovna
- Vorobeva Tatyana Vladimirovna
- Levandovskaya Kristina Georgievna
- Pavlenko Daniil Mikhajlovich
- Zinchenko Aleksej Alekseevich
- Miroshnikov Anatolij Ivanovich
- Kostromina Mariya Andreevna
- Esipov Roman Stanislavovich
|
RU2700452C1 |
| EXPRESSION PLASMID VECTOR OF MONOCISTRONIC EXPRESSION OF RECOMBINANT PROTEINS IN MAMMALIAN CELLS, CELL LINE OF MAMMALS-PRODUCERS OF RECOMBINANT PROTEIN, METHOD OF OBTAINING RECOMBINANT PROTEIN |
2013 |
- Orlova Nadezhda Aleksandrovna
- Kovnir Sergej Vladimirovich
- Vorob'Ev Ivan Ivanovich
|
RU2552170C2 |
| GENE SET EXPRESSION INCLUDING sORF CONSTRUCTS AND METHODS FOR IMMUNOGLOBULIN EXPRESSION |
2006 |
- Karson Dzheral'D R.
- Gion Uehndi
- Zal'Feld Jokhen G.
- Gu Tszitsze
- Redzhir Din A.
- K'Junz Jun
|
RU2478709C2 |
| EXPRESSION PLASMID VECTOR FOR HETEROLOGICAL EXPRESSION OF RECOMBINANT PROTEINS, HIGH-FREQUENCY INTEGRATION AND AMPLIFIED AMPLIFICATION OF EXPRESSION CASSETTE IN CELLS OF MAMMALS, BICISTRONIC MESSENGER RIZIFORM, METHOD OF PRODUCTION OF STABLE LINES OF PRODUCENTS OF RECOMBINANT PROTEINS USING SPECIFIED VECTOR, METHOD FOR PRODUCTION OF RECOMBINANT PROTEINS |
2011 |
- Orlova Nadezhda Aleksandrovna
- Kovnir Sergej Vladimirovich
- Vorob'Ev Ivan Ivanovich
|
RU2488633C1 |
| GENETIC CONSTRUCT CODING STRUCTURE OF T-CELL CHIMERIC RECEPTOR BASED ON SINGLE-CHAIN VHH ANTIBODIES SPECIFIC TO TUMOUR RECEPTOR CD47 FOR THE TARGETED IMMUNOTHERAPY OF MALIGNANT GROWTHS |
2017 |
- Chumakov Stepan Petrovich
- Kravchenko Yuliya Evgenevna
- Kravchenko Dmitrij Sergeevich
- Frolova Elena Ivanovna
|
RU2707535C2 |
| METHOD OF OBTAINING SYSTEM OF DIRECTED DELIVERY OF PROTEIN MOLECULES (ONCOLYTIC PROTEINS) INTO TUMOUR CELLS BASED ON ACTIVATED LYMPHOCYTES |
2013 |
- Chumakov Stepan Petrovich
- Kravchenko Julija Evgen'Evna
- Lezhnin Jurij Nikolaevich
- Frolova Elena Ivanovna
|
RU2552609C1 |
| PEPTINOGLYCANE HYDROLASE, EXPRESSION PLASMID, CONTAINING PEPTIDOGLYCANE HYDROLASE-CODING DNA FRAGMENT, BACTERIUM-PRODUCER AND METHOD OF MICROBIOLOGICAL SYNTHESIS OF PEPTIDOGLYCANE HYDROLASE |
2012 |
- Legotskij Sergej Aleksandrovich
- Miroshnikov Konstantin Anatol'Evich
- Kabanov Aleksandr Viktorovich
- Kljachko Natal'Ja L'Vovna
|
RU2547584C2 |
