METHOD OF PRODUCING RECOMBINANT NEURAMINIDASE NanH FROM CLOSTRIDIUM PERERINGENS Russian patent published in 2019 - IPC C12N15/00 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology, namely to methods for extraction and purification of recombinant proteins, in particular to production of highly purified preparation of recombinant neuraminidase NanH. Preparation of active, chromatograph homogenous with low content of bacterial endotoxins and host DNA preparation of NanH on an industrial scale is ensured by a chromatographic purification scheme including metal chelate chromatography and separation chromatography. NanH neuroanidase is obtained based on an optimized synthetic DNA sequence without removing a short sequence of six histidines contained at the N-terminus of the protein, which simplifies purification and eliminates the use of an expensive thrombin from the purification scheme. Already at the first stage of metal chelate chromatography, maximum purification of target protein from contaminant impurities of cell proteins takes place.

EFFECT: invention enables to obtain a high output of the target protein compared to the methods used previously: at least 1 g with 1 l of the culture liquid.

9 cl, 9 dwg, 5 tbl, 9 ex