METHOD OF PRODUCING LACTIC ACID Russian patent published in 2019 - IPC C12P7/56 C12N1/20 C12R1/225 

FIELD: biotechnologies.

SUBSTANCE: invention relates to the biotechnology. Lactic acid production method envisages introduction of bacteria of the genus Lactobacillus into a nutrient medium containing syrupy sterilized beet molasses, waste water pre-purified with the help of Chlorella vulgaris biomass at specified ratio of components, and culturing the lactic acid bacteria for 24–35 hours, at temperature of 37–50 °C, initial level of pH 6.5–7.5, while stirring 50–80 rpm, aeration of suspension at accumulation stage with gas-air mixture 60–80 l/h with subsequent provision of anaerobic conditions for 24–35 hours. In addition, in nutrient medium is treated with waste water purified to content of extracellular metabolites, mol/l: water-soluble vitamins B2, B12 – 10^-4–10^-7, soluble polysaccharides (galactose, arabinose, galactose, xylose) – 10^-4–10^-6, free fatty acids (stearic, oleic, linoleic, linolenic) – 10^-5–10^-7, polypeptides, free amino acids – 10^-5–10^-6, substances of phenolic nature (flavonoids) – 10^-6–10^-7, phytohormone: indolyl-3-acetic acid – 10^-5–10^-6.

EFFECT: invention increases output of lactic acid.

1 cl, 1 dwg, 5 tbl