METHOD FOR PRESERVATION OF LACTOBACILLUS DELBRUECKII Lactic acid bacteria Russian patent published in 2013 - IPC C12N1/04 C12N1/20 C12R1/225 

FIELD: food industry.

SUBSTANCE: method envisages bacteria suspension production in a liquid nutrient medium, the bacteria separation from the suspension by way of centrifugation to produce a biomass, the produced biomass mixing with a protective medium containing sucrose and glycerine and the produced mixture freezing at a temperature of (-18)-(-20)°C. The liquid nutrient medium contains components at the following ratio, wt %: (10 - 12) - sucrose, (3 - 5) - malt sprouts and (4 - 6) - chalk. Before freezing the biomass mixture with the protective medium is cooled at a temperature of 4-6°C, maintained at the said temperature during 1 hour.

EFFECT: enhancement of biosynthetic activity of Lactobacillus delbrueckii lactic acid bacteria during preservation by freezing and subsequent storage.

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